Review



cleaved cas9 protein  (GE Healthcare)


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    Structured Review

    GE Healthcare cleaved cas9 protein
    FnCas9 shows different cleavage kinetics for substrates as compared to SpCas9. In vitro cleavage assay showing activity of wild-type SpCas9 and wild-type FnCas9 (50 nM <t>Cas9:150</t> nM sgRNA) on indicated substrates (linearized plasmids, 250 ng) at different time points (1 min to 2 h). The top band represents uncleaved target while the 2 bottom bands represent cleaved products. Quantification is shown, Right. The y axis represents percentage cleavage. Error bars represent SD (3 independent experiments, C, control).
    Cleaved Cas9 Protein, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 21429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved cas9 protein/product/GE Healthcare
    Average 97 stars, based on 21429 article reviews
    cleaved cas9 protein - by Bioz Stars, 2026-02
    97/100 stars

    Images

    1) Product Images from "Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing"

    Article Title: Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1818461116

    FnCas9 shows different cleavage kinetics for substrates as compared to SpCas9. In vitro cleavage assay showing activity of wild-type SpCas9 and wild-type FnCas9 (50 nM Cas9:150 nM sgRNA) on indicated substrates (linearized plasmids, 250 ng) at different time points (1 min to 2 h). The top band represents uncleaved target while the 2 bottom bands represent cleaved products. Quantification is shown, Right. The y axis represents percentage cleavage. Error bars represent SD (3 independent experiments, C, control).
    Figure Legend Snippet: FnCas9 shows different cleavage kinetics for substrates as compared to SpCas9. In vitro cleavage assay showing activity of wild-type SpCas9 and wild-type FnCas9 (50 nM Cas9:150 nM sgRNA) on indicated substrates (linearized plasmids, 250 ng) at different time points (1 min to 2 h). The top band represents uncleaved target while the 2 bottom bands represent cleaved products. Quantification is shown, Right. The y axis represents percentage cleavage. Error bars represent SD (3 independent experiments, C, control).

    Techniques Used: In Vitro, Cleavage Assay, Activity Assay



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    cleaved cas9 protein  (GE Healthcare)
    97
    GE Healthcare cleaved cas9 protein
    FnCas9 shows different cleavage kinetics for substrates as compared to SpCas9. In vitro cleavage assay showing activity of wild-type SpCas9 and wild-type FnCas9 (50 nM <t>Cas9:150</t> nM sgRNA) on indicated substrates (linearized plasmids, 250 ng) at different time points (1 min to 2 h). The top band represents uncleaved target while the 2 bottom bands represent cleaved products. Quantification is shown, Right. The y axis represents percentage cleavage. Error bars represent SD (3 independent experiments, C, control).
    Cleaved Cas9 Protein, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved cas9 protein/product/GE Healthcare
    Average 97 stars, based on 1 article reviews
    cleaved cas9 protein - by Bioz Stars, 2026-02
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    cleaved cas9 protein  (Danaher Inc)
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    Danaher Inc cleaved cas9 protein
    FnCas9 shows different cleavage kinetics for substrates as compared to SpCas9. In vitro cleavage assay showing activity of wild-type SpCas9 and wild-type FnCas9 (50 nM <t>Cas9:150</t> nM sgRNA) on indicated substrates (linearized plasmids, 250 ng) at different time points (1 min to 2 h). The top band represents uncleaved target while the 2 bottom bands represent cleaved products. Quantification is shown, Right. The y axis represents percentage cleavage. Error bars represent SD (3 independent experiments, C, control).
    Cleaved Cas9 Protein, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    FnCas9 shows different cleavage kinetics for substrates as compared to SpCas9. In vitro cleavage assay showing activity of wild-type SpCas9 and wild-type FnCas9 (50 nM Cas9:150 nM sgRNA) on indicated substrates (linearized plasmids, 250 ng) at different time points (1 min to 2 h). The top band represents uncleaved target while the 2 bottom bands represent cleaved products. Quantification is shown, Right. The y axis represents percentage cleavage. Error bars represent SD (3 independent experiments, C, control).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing

    doi: 10.1073/pnas.1818461116

    Figure Lengend Snippet: FnCas9 shows different cleavage kinetics for substrates as compared to SpCas9. In vitro cleavage assay showing activity of wild-type SpCas9 and wild-type FnCas9 (50 nM Cas9:150 nM sgRNA) on indicated substrates (linearized plasmids, 250 ng) at different time points (1 min to 2 h). The top band represents uncleaved target while the 2 bottom bands represent cleaved products. Quantification is shown, Right. The y axis represents percentage cleavage. Error bars represent SD (3 independent experiments, C, control).

    Article Snippet: The cleaved Cas9 protein was separated from fusion tag on HiLoad Superdex 200 16/60 column (GE Healthcare) in 20 mM HEPES pH 7.5, 150 mM KCl, 10% glycerol, 1 mM DTT.

    Techniques: In Vitro, Cleavage Assay, Activity Assay